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• Pires de Miranda M, Quendera AP, McVey CE, Kaye KM, Simas JP. (2018) "In Vivo Persistence of Chimeric Virus after Substitution of the Kaposi's Sarcoma-Associated Herpesvirus LANA DNA Binding Domain with That of Murid Herpesvirus 4." J Virol. 92(21). pii: e01251-18. doi:  [10.1128/JVI.01251-18]

• M.C. Almadanim, N.M. Gonçalves, M.T.G. Rosa, B.M. Alexandre, A.M. Cordeiro, M. Rodrigues, N.J. Saibo, C.M. Soares, C.V. Romão, M. Oliveira, and I.A. Abreu I. (2018) "The rice cold-responsive calcium-dependent protein kinase OsCPK17 is regulated by alternative splicing and post-translational modifications" BBA- Mol Cell Res 1865 (2): 231-246.

C. da Silveira Tomé, A.E. Foucher, J.M. Jault, D. Housset (2017) "High concentrations of GTP induce conformational changes in the essential bacterial GTPase EngA and enhance its binding to the ribosome", FEBS J 285(1):160-177.


• F.M. Sousa, F.V. Sena, A.P. Batista, D. Athayde, J.A. Brito, M. Archer, A.S.F. Oliveira, C.M. Soares, T. Catarino, and M.M. Pereira (2017) "The key role of glutamate 172 in the mechanism of type II NADH:quinone oxidoreductase of Staphylococcus aureus", Biochim Biophys Acta 1858(10): 823-832.

• A.C. Habison, M.P. de Miranda, C. Beauchemin, M. Tan, S.A. Cerqueira, B. Correia, R. Ponnusamy, E.J. Usherwood, C.E. McVey, J.P. Simas, and K.M. Kaye (2017) "Cross-species conservation of episome maintenance provides a basis for in vivo investigation of Kaposi's sarcoma herpesvirus LANA", PLoS Pathog 13(9): e1006555.

E. Moe, F. Rollo, C.M. Silveira, M. Sezer, P. Hildebrandt, and S. Todorovic (2017) "Spectroelectrochemical insights into structural and redox properties of immobilized endonuclease III and its catalytically inactive mutant", Spectrochim Acta A Mol Biomol Spectrosc, in press

L. Pereira, I.H. Saraiva, A.S.F. Oliveira, C.M. Soares, R.O. Louro, and Frazão C (2017) "Molecular structure of FoxE, the putative iron oxidase of Rhodobacter ferrooxidans SW2", Biochim Biophys Acta 1858(10):847-853.

M. Alves, N.S.M. Vieira, L.P.N. Rebelo, J.M.M. Araújo, A.B. Pereiro, and M. Archer (2017) "Fluorinated ionic liquids for protein drug delivery systems: Investigating their impact on the structure and function of lysozyme", Int J Pharm 526(1-2): 309-320.

M.C. Marques, C. Tapia, O. Gutiérrez-Sanz, A.R. Ramos, K.L. Keller, J.D. Wall, A.L. De Lacey, P.M. Matias, and I.A.C. Pereira (2017) "The direct role of selenocysteine in [NiFeSe] hydrogenase maturation and catalysis", Nat Chem Biol 13(5): 544-550.

• E. Forte, V.B. Borisov, J.B. Vicente, and A. Giuffrè (2017) "Cytochrome bd and Gaseous Ligands in Bacterial Physiology", Adv Microb Physiol 71: 171-234.

S.P. Santos, M.G. Cuypers, A. Round, S. Finet, T. Narayanan, E.P. Mitchell, and C.V. Romão (2017) "SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions", J Mol Biol  429(5): 667-687.

• A.I. Coelho, E. Rubio-Gozalbo, J.B. Vicente, and I.A. Rivera (2017) "Sweet and sour: "Sweet and sour: an update on classic galactosemia", J Inherit Metab Dis 40(3): 325-342.

• T.L. Clarke, M.P. Sanchez-Bailon, K. Chiang, J.J. Reynolds, J. Herrero-Ruiz, T.M. Bandeiras, P.M. Matias, S.L. Maslen, J.M. Skehel, G.S. Stewart, and C.C. Davies (2017) "PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination", Mol Cell 65(5): 900-916.e7.

J.B. Vicente, H.G. Colaço, F. Malagrinò, P.E. Santo, A. Gutierres, T.M. Bandeiras, P. Leandro, J.A. Brito, and A. Giuffrè (2017) "A clinically relevant variant of the human hydrogen sulfide-synthesizing enzyme cystathionine β-synthase: increased CO reactivity as a novel molecular mechanism of pathogenicity?", Oxid Med Cell Longev 2017: 8940321.


• E.F. Ruivo, L.M. Gonçalves, L.A. Carvalho, R.C. Guedes, S. Hofbauer, J.A. Brito, M. Archer, R. Moreira and S.D. Lucas (2016) "Clickable 4-Oxo-β-lactam-Based Selective Probing for Human Neutrophil Elastase Related Proteomes", ChemMedChem 11(18): 2037-42

• S.A. Cerqueira, M.Tan, S. Li, F. Juillard, C.E. McVey, K.M. Kaye, J.P. Simas (2016) "LANA E3 Ubiquitin-Ligase Activity Impacts Gammaherpesvirus-Driven Germinal Center B Cell Proliferation", J Virol. 2016 Jun 15.

J.B. Vicente, F. Malagrinò, M. Arese, E. Forte, P. Sarti, and A. Giuffrè* (2016) " Bioenergetic relevance of hydrogen sulfide and the interplay between gasotransmitters at human cystathionine -synthase", BBA Bioenergetics, in press

J.M. Kurth, J.A. Brito, J. Reuter, A. Flegler, T. Koch, T. Franke, E.M. Klein, S.F. Rowe, J.N. Butt, K. Denkmann, I.A. Pereira, M. Archer and C. Dahl (2016) "Electron Accepting Units of the Diheme Cytochrome c TsdA, a Bifunctional Thiosulfate Dehydrogenase/Tetrathionate Reductase", J Biol Chem. 291(48):24804-24818

• E. Forte, V. Borisov, M. Falabella, H.G. Colaço, M. Tinajero-Trejo, R. Poole, J.B. Vicente, P. Sarti, and A. Giuffrè (2016) "The Terminal Oxidase Cytochrome bd Promotes Sulfide-resistant Bacterial Respiration and Growth", Sci Reports, in press

• H.G. Colaço, P.E. Santo, P.M. Matias, T.M. Bandeiras, J.B. Vicente (2016) "Roles of Escherichia coli ZinT in cobalt, mercury and cadmium resistance and structural insights into the metal binding mechanism" Metallomics 8:327-336.

C.V. Romão, J.B. Vicente, C. Frazão, and M. Teixeira (2016) "The dual function of flavodiiron proteins: oxygen and/or nitric oxide reductases", J Biol Inorg Chem 21(1): 39-52.

J.B. Vicente, H.G. Colaço, P. Sarti, P. Leandro, and A. Giuffrè* (2016) "S-Adenosyl-L-methionine modulates CO and NO· binding to the human H2S-generating enzyme cystathionine ß-synthase", J Biol Chem 291(2): 572-81.



R. Ponnusamy, M.V. Petoukhov, B. Correia, T.F. Custodio, F. Juillard, M. Tan, M. Pires de Miranda, M.A. Carrondo, J.P. Simas, K.M. Kaye, D.I. Svergun, C.E. McVey (2015) "KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA." Nucleic Acids Res. 43 (20): 10039-54.

J. A. Brito, K. Denkmann, I. A. C. Pereira, M. Archer, C. Dahl (2015), “Thiosulfate Dehydrogenase (TsdA)  from Allochromatium vinosum: Structural and Functional Insights into Thiosulfate Oxidation”, J. Biol. Chem. 290(14):9222-38.

• N. Zaarur, X. Xu, P. Lestienne, A.B. Meriin, M. McComb, C.E. Costello, G.P. Newnam, R. Ganti, N.V. Romanova, M. Shanmugasundaram, S.T. Silva, T.M. Bandeiras, P.M. Matias, K.S. Lobachev, I.K. Lednev, Y.O. Chernoff, M.Y. Sherman (2015) “RuvbL1 and RuvbL2 enhance aggresome formation and disaggregate amyloid fibrils” EMBO Journal 112(37):11455-11460.

C.M. Sousa, P. Carpentier, P.M. Matias, F. Testa, F. Pinho, P. Sarti, A. Giuffrè, T.M. Bandeiras, C.V. Romão (2015) “Superoxide reductase from Giardia intestinalis: structural characterization of the first SOR from a eukaryotic organism shows an iron centre highly sensitive to photo-reduction” Acta Crystallographica D 71:2236-2247.

• Pedersen, H. L., Johnson, K. A., McVey, C. E., Leiros, I. &, Moe E. (2015). “Structure determination of uracil-DNA N-glycosylase from Deinococcus radiodurans in complex with DNA”. Acta Crystallogr D Biol Crystallogr 71: 2137-49

Moe, E., Assefa, N. G., Leiros, I., Torseth, K. J., Smalås, A. O. & Willassen, N. P. (2015). “Reduced Hydrophobicity of the Minor Groove Intercalation Loop is Critical for Efficient Catalysis by Cold Adapted Uracil-DNA N-Glycosylase from Atlantic Cod”. J Thermodyn Catal 6:155

A. L. Rosário, F. V. Sena, A. P. Batista, T. F. Oliveira, D. Athayde, M. M. Pereira, J. A. Brito, M. Archer (2015), “Expression, purification, crystallization and preliminary X-ray diffraction analysis of a type II NADH:quinone oxidoreductase from the human pathogen Staphylococcus aureus”, ActaCryst.F 71(4),477-482.

• F. V. Sena, A. P. Batista, T. Catarino, J. A. Brito, M. Archer, M. Viertler, T. Mandl, E. J. Cabrita, M. M. Pereira (2015), “Type-II NADH:quinone oxidoreductase from Staphylococcus aureus has two distinct binding sites and is rate limited by quinone reduction”,  Mol. Microbiol. 98(2):272-88.

• C. G. Fernandes, D. Plácido, D. Lousa, J. A. Brito, A. Isidro, C. M.  Soares, J. Pohl, M. A. Carrondo, M. Archer, A. O. Henriques (2015, “Structural and Functional Characterization of an Ancient Bacterial Transglutaminase Sheds Light on the Minimal Requirements for Protein Cross-Linking”, Biochemistry 54(37):5723-34.

S. HofbauerJ. A. Brito,  J. Mulchande,  P. NoglyM. Pessanha,  R. Moreira, M. Archer (2015), “Stabilization of Porcine Pancreatic Elastase (PPE) crystals by glutaraldehyde cross-linking”, ActaCryst.F 71(10),1346-51.

• S. Li, M. Tan, F. Juillard, R. Ponnusamy, B. Correia, J. P. Simas, M.A. Carrondo, C.E. McVey, K.M. Kaye. (2015) “The Kaposi's sarcoma herpesvirus latency-associated nuclear antigen DNA binding domain dorsal positive electrostatic patch facilitates DNA replication and episome persistence.” J Biol Chem 290(47): 28084-96

P.M. Matias, S.H. Baek, T.M. Bandeiras, A. Dutta, W.A. Houry, O. Llorca, J. Rosenbaum (2015) “The AAA+ proteins Pontin and Reptin enter adult age: From understanding their basic biology to the identification of selective inhibitors” Frontiers in Molecular Biosciences 2:17.

• J.P. Leite, M. Duarte, A.M. Paiva, F. Ferreira-da-Silva, P.M. Matias, O.C. Nunes, L. Gales (2015) “Structure-guided engineering of molinate hydrolase for the degradation of thiocarbamate pesticides” PLoS One 10:e0123430.

M.I. Mendes, D.E. Smith, J.B. Vicente, I. Tavares De Almeida, T. Ben-Omran, G.S. Salomons, I.A. Rivera, P. Leandro, H.J. Blom (2015) "Small aminothiol compounds improve the function of Arg to Cys variant proteins: effect on the human cystathionine ß-synthase p.R336C", Hum Mol Genet 24(25): 7339-48.

• Sarre, A., Ökvist, M., Klar, T., Hall, D., Smalas, A., McSweeney, S., Timmins, J. &, Moe E. (2015). «Structure/function studies of two unusual Endonuclease III enzymes from the extreme radiation and desiccation resistant bacterium Deinococcus radiodurans”. J Struct Biol. 191: 87-99

Niiranen, L., Lian, K., K., J. & Moe, E. (2015). «Crystal structure determination of DNA polymerase III b-subunit from the extreme radiation and desiccation resistant bacterium Deinococcus radiodurans”. BMC structural biology 15:5

Lian, K., Leiros, H. K. & Moe, E. (2015). “MutT from the fish pathogen Aliivibrio salmonicida is a cold-active nucleotide-pool sanitization enzyme with unexpectedly high thermostability”. FEBS Open Bio 5: 107-16

Moe, E., Sezer, M., Hildebrandt, P. & Todorovic, S. (2015). “Surface enhanced vibrational spectroscopic evidence for an alternative DNA-independent redox activation of endonuclease III”. Chem Commun (Camb) 51: 3255-7




• J.A. Brito, A. Gutierres, K. Denkmann, C. Dahl and M. Archer, "Production, crystallisation and premilinary crystallographic analysis of Allochromatium vinosum thiosulfate dehydrogenase TsdA, an unusual acidophilic c-type cytochrome", Acta F. (2014), F70(10): 1424-1427.

• S.P. Miller, S. Gonçalves, P.M. Matias and A.M. Dean, "Evolution of a transition state: role of Lys100 in the active site of isocitrate dehydrogenase", ChemBioChem. (2014), 15(8): 1145-1153.

• N. Borges, C.D. Jorge, L.G. Gonçalves, S. Gonçalves, P.M. Matias and H. Santos, "Mannosglycerate: structural analysis of biosynthesis and evolutionary history", Extremophiles. (2014), 18(5): 835-852.

• P. Nogly, I. Guschin, A. Remeeva, A.M. Esteves, N. Borges, P. Ma, A. Ishchenko, S. Grudinin, E. Round, I. Moraes, V. Borshchevskiy, H. Santos, V. Gordeliy and M. Archer "X-ray structure of a CDP-alcohol phosphatidyltransferase membrane enzyme and insights into its catalytic mechanism", Nature Commun. (2014), 5: 4169.

• P.T. Borges, C. Frazão, C.S. Miranda, M.A. Carrondo, and C.V. Romão, "Structure of the mono-functional heme catalase DR1998 from Deinococcus radiodurans", FEBS J. (2014), 281(18): 4138-4150.

• P.T. Borges, C.S. Miranda, S.P. Santos, J.N. Carita, C. Frazão and C.V. Romão, "Purification, crystallization and phase determination of the DR1998 haem b catalase  from Deinococcus radiodurans", Acta Cryst. (2014) F70(5): 659-662.

• A.T. Marques, S.P. Santos, C.S. Miranda, M.J. Rosa, M.A. Rodrigues, I.A. Abreu, C. Frazão and C.V. Romão, "Expression, purification and crystallization of MnSOD from Arabidopsis thaliana", Acta Cryst. (2014) F70(5): 669-672.

• C.E. McVey, M.J. Ferreira, B. Correia, S. Lahiri, D. de Sanctis, M.A. Carrondo, P.F. Lindley, I. de-Sá-Nogueira, C.M. Soares and I. Bento, "The importance of the Abn2 calcium cluster in the endo-1,5-arabinanase activity from Bacillus subtilis", JBIC. (2014), 19: 505-513.



• A.M. Gonçalves, S. Mendes, D. de Sanctis, L.O. Mendes and I. Bento, "The Crystal Structure of Pseudomonas putida Azoreductase: the active site revisited." , FEBS J. (2013), 280(24):6643-6657.

• B. Correia, S.A. Cerqueira, C. Beauchemin, M. Pires de Miranda, S. Li, R. Ponnusamy, L. Rodrigues, T. R. Schneider, M. A. Carrondo, K. M. Kaye, J. P. Simas and C. E. McVey “Crystal structure of the gamma-2 herpesvirus LANA DNA binding domain identifies charged surface residues which impact viral latency", PLoS Pathog. (2013) 9(10): e1003673. doi:10.1371/journal.ppat.1003673.

• M. Pires de Miranda, F. B. Lopes, C. E. McVey, X. R. Bustelo,and J. P. Simas.
"Role of Src-homology domain binding in signaling complexes assembled by the murid gammaherpesvirus M2 protein." J. Biol. Chem. 2013 vol. 288 (6) p. 3858-3870.

• P. Ma, F. Varela, M. Magoch, A.R. Silva, A.L. Rosario, J. Brito, T. Oliveira, P. Nogly, M. Pessanha, M. Stelter, A. Kletzin, P.J.F. Henderson and M. Archer, "An efficient strategy for small-scale screening and production of archaeal membrane transport proteins in Escherichia coli." PLoSOne (2013), 8(10): e76913. doi: 10.1371/journal.pone.0076913.

• P. Nogly, P.M. Matias, M. de Rosa, R. Castro, H. Santos, A.R. Neves and M. Archer “High-resolution structure of an atypical a-phosphoglucomutase related to eukaryotic phosphomannomutases", Acta Cryst. D (2013), 69(10): 2008-2016. doi: 10.1107/S0907444913017046

• M.A. Sharkey, T.F. Oliveira, P.C. Engel, and A.R. Khan, "Structure of NADP(+)-dependent glutamate dehydrogenase from Escherichia coli - reflections on the basis of coenzyme specificity in the family of glutamate dehydrogenases", FEBS J. (2013), 280(18):4681-4692.

• M.L.Deb, S.S. Dey, I. Bento, M.T. Barros and C.D. Maycock, "Copper-Catalyzed Regioselective Intramolecular Oxidative α-Functionalization of Tertiary Amines: An Efficient Synthesis of Dihydro-1,3-Oxazines", Angew. Chem. Intl. Ed. (2013), 52(37):9791-9795.

• M.C. Marques, R. Coelho, I.A.C. Pereira, P.M. Matias, "Redox state-dependent changes in the crystal structure of [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough"  Int. J. Hydrogen Energ. (2013) 38(21):8664–8682. doi: 10.1016/j.ijhydene.2013.04.132.

• J. Rosenbaum, S.H. Baek, A. Dutta, W.A. Houry, O. Huber, T.R. Hupp,  P.M. Matias, "The Emergence of the Conserved AAA+ ATPases Pontin and Reptin on the Signaling Landscape"  Sci. Signal. (2013) 6:mr1.

•T.M Bandeiras, M.C. Freitas, D. Petrasch, A. Kletzin and C. Frazão, “SAD phasing towards structure determination of thermostable Rieske ferrodoxin with a novel stabilizing disulfide bridge”,  Acta Cryst. (2013), F69(5): 555-558.

•A. Barbas, A. Popescu, C. Frazão, C.M. Arraiano and A. Fialho, “Rossman-fold motifs can confer multiple functions to metabolic enzymes: RNA binding and ribonuclease activity of a UDP-glucose dehydrogenase”,  Biochem. Biophys. Res. Comm. (2013), 430(1): 218-224.



•Z. Carvalho-Santos, P. Machado, I. Alvarez-Martins, S.M. Gouveia, S.C. Jana, P. Duarte, T. Amado, P. Branco, M.C. Freitas, S.T. Silva, C. Antony, T.M. Bandeiras and M. Bettencourt-Dias, “BLD19/CEP135 is a microtubule-associated protein that controls the formation of the flagellum central microtubule pair”,  Dev. Cell (2012), 23(2): 412-424.

• S. Gonçalves, S.P. Miller, M.A. Carrondo, A.M. Dean and P.M. Matias, "Induced Fit and the Catalytic Mechanism of Isocitrate Dehydrogenase", Biochemistry (2012), 51:7098–7115. 

• A.M. Gonçalves, C.S. Silva, T.I. Madeira, R. Coelho, D. de Sanctis, M.V. San Romão and I. Bento, "Endo-beta-D-1,4-mannanase from Chrysonilia sitophila displays a novel loop arrangement for substrate selectivity", Acta Cryst. (2012), D68, 1468-1478.

• C.N. Santos, M.M. Alves, I. Bento and Ricardo B. Ferreira "Missing pieces in protein deposition and mobilization inside legume seed storage vacuoles: calcium and magnesium ions",  Seed Science Research (2012), in press

• L. Pereira, I.H. Saraiva, R. Coelho, D.K. Newman, R.O. Louro and C. Frazão,“Crystallisation and preliminary crystallographic studies of FoxE from Rhodobacter ferrooxidans SW2, an Fe(II) oxidoreductase involved in photoferrotrophy” , Acta Cryst. (2012), F68(9): 1106-1108.

• P. Nogly, R. Castro, M. de Rosa, A.R. Neves, H. Santos and M. Archer, “Production and crystallisation of alpha-glucomutase from Lactococcus lactis”, Acta Cryst. (2012), F68(9): 1113-1115.

• A.R. Marques, L. Kromer, D.J. Gallo, N. Penacho, S.S. Rodrigues, J.D. Seixas, G.J.L. Bernardes, P.M. Reis, S.L. Otterbein, R.A. Ruggieri, A.S.G. Gonçalves, A.M.L. Gonçalves, M.N. De Matos, I. Bento, L.E. Otterbein, W.A. Blättler, and C.C. Romão, “Generation of Carbon Monoxide Releasing Molecules (CO-RMs) as Drug Candidates for the Treatment of Acute Liver Injury: Targeting of CO-RMs to the Liver”, Organometallics (2012), 31 (16): 5810–5822.

• S.P. Santos, T.M. Bandeiras, A.F. Pinto, M. Teixeira, M.A. Carrondo, C.V. Romão, “Thermofluor-based optimisation strategy for the stabilsation and crystallisation of Campylobacter jejuni desulforubrerythrin”,  Protein Exp. Purif. (2012), 81(2): 193-200.

• C.S. Silva, J.M. Damas, Z. Chen, V. Brissos, L.O. Martins, C.M. Soares, P.F. Lindley and I. Bento, “The role of Asp116 in the reductive cleavage of dioxygen to water in CotA laccase: assistance during the proton-transfer mechanism ”, Acta Cryst. (2012), D68(2): 186-193.

• C.S. Silva, P. Durão, A. Fillat, P.F. Lindley, L.O. Martins, I. Bento, “Crystal structure of the multicopper oxidase from the pathogenic bacterium Campylobacter jejuni CGUG11284: characterization of a metallo-oxidase”, Metallomics (2012), 4(1): 37-47. (Front cover


Book Chapters:

• C.V. Romão, M. Archer, S.A. Lobo, R.O. Louro, I.A.C. Pereira, L.M. Saraiva, M. Teixeira and P.M. Matias "Diversity of Heme Proteins in Sulfate Reducing Bacteria" in HANDBOOK OF PORPHYRIN SCIENCE - With Applications to Chemistry, Physics, Materials Science, Engineering, Biology and Medicine (2012) (K.M. Kadish, K.M. Smith and R. Guilard, eds.), 19(89), pp 139-230, World Scientific Publishing Co. Pte. Ltd., Singapore.


• M.A. Carrondo and P. Spadon (eds) “Macromolecular Crystallography: Deciphering the structure, function and dynamics of biological molecules”, NATO Science for Peace and Security Series A: Chemistry and Biology (2012),Springer: Dordrecht (ISBN 978-94-007-2529-4) XIV, 208 p. 95 illus. DOI: 10.1007/978-94-007-2530-0.


• S. Gorynia, T.M. Bandeiras, P.M. Matias, F.G. Pinho, C. McVey, P. Donner and M.A. Carrondo, “RuvBL1 and RuvBL2 and their complex proteins implicated in many cellular pathways”, in Macromolecular Crystallography: Deciphering the structure, function and dynamics of biological molecules (2012), (M.A Carrondo and P. Spadon, eds) NATO Science for Peace and Security Series A: Chemistry and Biology, pp. 55-63. DOI: 10.1007/978-94-007-2530-0_5.



• S. Gonçalves, A.M. Esteves, H. Santos, N. Borges and P.M. Matias, “The three-dimensional structure of mannosyl-3-phosphoglycerate phosphatase from Thermus thermophilus HB27: a new member of the Haloalcanoic Acid Dehalogenase Superfamily”, Biochemistry (2011), 50(44): 9551-9567.

• J. Rocha, A.O. Popescu, P. Borges, D. Mil-Homens, L.M. Moreira, I. Sá-Correia, A.M. Fialho and C. Frazão, “Structure of Burkholderia cepacia UDP-Glucose Dehydrogenase (UGD) BceC and role of Tyr10 in final hydrolysis of UGD thioester intermediate”, J. Bacteriol. (2011), 193(15): 3978-3987.

• J.A. Brito, N. Borges, C. Vonrhein, H. Santos and M. Archer, “Crystal structure of Archaeoglobus fulgidus CTP:Inositol-1-Phosphate Cytidylyltransferase, a key enzyme for Di-myo-Inositol-Phosphate synthesis in (Hyper)thermophiles”, J. Bacteriol. (2011), 193(9): 2177-2185.

• F.G. Pinho, A.F. Pinto, L. Pinto, H. Huber, C.V. Romão, M. Teixeira,  P.M. Matias and  T.M. Bandeiras,  “Superoxide Reductase from Nanoarchaeum equitans: expression, purification, crystallization and preliminary X-ray crystallographic analysis”, Acta Cryst. (2011), F67(5): 591-595.

• T.F. Oliveira, E. Franklin, J.P. Afonso, A.R. Khan, N.J. Oldham, I.A.C. Pereira and M. Archer, “Structural insights into dissimilatory sulfite reductases: structure of desulforubidin from Desulfomicrobium norvegicum”, Front Microbiol. (2011),2:71.

• A.T. Fernandes, M.M. Pereira, C.S. Silva, P.F. Lindley, I. Bento, E.P. Melo and L.O. Martins, “The removal of a disulfide bridge in CotA-laccase changes the slower motion dynamics involved in copper binding but has no effect on the thermodynamic stability”, J. Biol. Inorg. Chem. (2011), 16(4): 641-651.

• A. Veith, T. Urich, K. Seyfarth, J. Protze, C. Frazão, A. Kletzin, “Substrate pathways and mechanisms of inhibition in the sulfur oxygenase reductase of Acidianus ambivalens”, Front. Microbio. (2011), 2(37).

• S. Gonçalves, A.M. Esteves, N. Borges, H. Santos and P.M. Matias, “Crystallization and preliminary X-ray analysis of mannosyl-3-phosphoglycerate phosphatase from Thermus thermophilus HB27”, Acta Cryst. (2011) F67(3): 390-396.

• C.S.A. Baltazar, M.C. Marques, C.M. Soares, A.M. DeLacey, I.A.C. Pereira and P.M. Matias, “Nickel-Iron-Selenium Hydrogenases - An Overview”, Eur. J. Inorg. Chem. (2011), 7: 948-962.

• A.F. Pinto, S. Todorovic, P. Hildebrandt, M. Yamazaki, F. Amano, S. Igimi, C.V. Romão and M. Teixeira, “Desulforubrerythrin from Campylobacter jejuni, a novel multi-domain protein”, J. Biol. Inorg. Chem. (2011), 16(3): 501-510.

• B. Correia, Z. Chen, S. Mendes, L.O. Martins and I. Bento, “Crystallization and preliminary X-ray diffraction analysis of the Azoreductase PpAzoR from Pseudomonas putida MET94”, Acta Cryst. (2011) F67(1): 121-123.

• C.V. Romão, D. Ladakis, S.A.L. Lobo, M.A. Carrondo, A.A. Brindley, E. Deery, P.M. Matias, R.W. Pickersgill, L.M. Saraiva and M.J. Warren, “Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization”, PNAS (2011), 108(1): 97-102.

• S. Gorynia, T.M. Bandeiras, F.G. Pinho, C.E. McVey, C. Vonrhein, A. Round, D.I. Svergun, P. Donner, P.M. Matias and M.A. Carrondo, “Structural and functional insights into a dodecameric molecular machine - the RuvBL1/RuvBL2 complex”, J. Struct. Biol. (2011), 176(3): 279-291.

• T.M. Bandeiras, S. Gorynia, C. Vonrhein, P. Donner, P.M. Matias and M.A. Carrondo, “Structural insights into a dodecameric molecular machine - the RuvBL1/RuvBL2 complex”, Acta Cryst. (2011). A67, C267 (Proceedings from the IUCR, Madrid, 2012)

• A. Athanasiadis, M. de Rosa, D. de Sanctis, A.L. Rosário, M. Archer, A. Rich and M.A. Carrondo, “The structure of conformational junctions in DNA and genomic instability”, Acta Cryst. (2011). A67, C147 (Proceedings from the IUCR, Madrid, 2012)

Book Chapters:

• J. B. Vicente, M. A. Carrondo, M. Teixeira and C. Frazão “Flavodiiron Proteins: Nitric Oxide and/or Oxygen Reductases”, in Encyclopedia of Inorganic and Bioinorganic Chemistry (2011), John Wiley & Sons, Ltd.

• I. Bento, R. O. Louro, C. M. Soares, M. A. Carrondo, “Tetraheme Cytochrome c3”, in Encyclopedia of Inorganic and Bioinorganic Chemistry (2011),  John Wiley & Sons, Ltd.


• D. de Sanctis, J.M. Inácio, P.F. Lindley, I. de Sá Nogueira and I. Bento, “New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases”, FEBS J. (2010) 277(21): 4562-4574.

• J.A. Brito, N. Borges, H. Santos and M. Archer, “Production, crystallization and preliminary X-ray analysis of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus”, Acta Cryst. (2010) F66(11): 1463-1465.

• I. Bento, C.S. Silva, Z. Chen, L.O. Martins, P.F. Lindley and C.M. Soares, “Mechanisms underlying dioxygen reduction in laccases. Structural and modelling studies focusing on proton transfer”, BMC Structural Biology (2010), 10(28): 1-14.

• S. Gonçalves, N. Borges, A.M. Esteves, B. Victor, C.M. Soares, H. Santos and P.M. Matias, “Structural analysis of Thermus thermophilus HB27 mannosyl-3-phosphoglycerate synthase provides evidence for a second catalytic metal ion and new insight into the retaining mechanism of glycosyltransferases”, J. Biol. Chem. (2010), 285(23): 17857-17868.

• M. de Rosa, D. de Sanctis, M. Archer, A. Rich, A. Athanasiadis and M.A. Carrondo, “Crystal structure of a junction between two Z-DNA helices”, PNAS (2010), 107(20): 9088-9092.

• F.G. Pinho, C.V. Romão, A.F. Pinto, L.M. Saraiva, H. Huber, P.M. Matias, M.A. Carrondo, M. Teixeira and T.M. Bandeiras, “Cloning, purification, crystallization and X-ray crystallographic analysis of Ignicoccus hospitalis neelaredoxin”, Acta Cryst. (2010) F66(5): 605-607.

• M. Stelter, A.M.P. Melo, G.O. Hreggvidsson, S. Hjorleifsdottir, L.M. Saraiva, M. Teixeira, M. Archer, “The structure at 1.0Å resolution of a high-potential iron-sulfur protein (HiPIP) involved in the aerobic respiratory chain of Rhodothermus marinus”, J. Biol. Inorg. Chem. (2010), 15(3): 303-313.

• M.C. Marques, R. Coelho, A.L. De Lacey, I.A.C. Pereira, P.M. Matias “The three-dimensional structure of [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough: a Hydrogenase without a bridging ligand in the active site in its oxidised, “as-isolated” state”, J. Mol. Biol. (2010), 396(4): 893-907.

• T.M. Bandeiras, C.V. Romão, J.V. Rodrigues, M. Teixeira, P.M. Matias, “Purification, crystallization and X-ray crystallographic analysis of Archaeoglobus fulgidus neelaredoxin”, Acta Cryst. (2010) F66(3): 316-319.

• J. Rocha, A.O. Popescu, I. Sá-Correia, A.M. Fialho and C. Frazão, “Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408”, Acta Cryst. (2010) F66(3): 269-271.

• Z. Chen, P. Durão, C.S. Silva, M.M. Pereira, S. Todorovic, P. Hildebrandt, I. Bento, P.F. Lindley and L.O. Martins, “The role of Glu498 in the dioxygen reactivity of CotA-laccase from Bacillus subtilis”, Dalton Trans. (2010) 39(11): 2875-2882.

• J. Rocha, A.T. Granja, I. Sá-Correia, A.M. Fialho and C. Frazão, “Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461”, Acta Cryst. (2010) F66(1): 69-72.



• P.B. Crowley, P.M. Matias, A.R. Khan, M. Roessle and D.I. Svergun “Metal-mediated self-assembly of a beta-Sandwich protein”, Chem. Eur. J. (2009), 15(46): 12672-12680.

• S. Gonçalves, N. Borges, H. Santos and P.M. Matias “Crystallization and preliminary X-ray analysis of mannosyl-3-phosphoglycerate synthase from Thermus thermophilus HB27”, Acta Cryst. (2009) F65(10), 1014–1017.

• M. Marques, R. Coelho, I.A.C. Pereira and P.M. Matias “Purification, crystallization and preliminary crystallographic analysis of the [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough”, Acta Cryst. (2009) F65(9), 920–922.

• A.T. Gonçalves, D. Marçal, M.A. Carrondo and F.J. Enguita “Crystallization and preliminary X-ray characterization of a glycerol dehydrogenase from the human pathogen Salmonella enterica serovar Typhimurium”, Acta Cryst. (2009) F65(7), 698–701.

• J.A. Brito, F.L. Sousa, M. Stelter, T.M. Bandeiras, C. Vonrhein, M. Teixeira, M.M. Pereira and M. Archer “Structural and functional insights into sulfide:quinone oxidoreductase” Biochemistry (2009), 48(24): 5613-5622.

• D. de Sanctis, C.E. McVey, F.J. Enguita and M.A. Carrondo “Crystal structure of the full-length sorbitol operon regulator, SorC from Klebsiella pneumoniae: structural evidence for a novel transcriptional regulation mechanism” J. Mol. Biol. (2009), 387(3): 759-770.

• D. Marçal, A.T. Rêgo, M.A. Carrondo and F.J. Enguita “1,3-propanediol-dehydrogenase from Klebsiella pneumoniae: decameric quaternary structure and possible subunit cooperativity” J. Bacteriol. (2009), 191(4): 1143-1151.

• T.M. Bandeiras, P.N. Refojo, S. Todorovic, D.H. Murgida, P. Hildebrandt, C. Bauer, M.M. Pereira, A. Kletzin and M. Teixeira, “The cytochrome ba complex from the thermoacidophilic crenarchaeote Acidianus ambivalens is an analog of bc1 complexes” Biochimica et Biophysica Acta – Bioenergetics (2009), 1787(1): 37-45.

• L. Toussaint, M.G. Cuypers, L. Bertrand, L. Hue, C.V. Romão, L.M. Saraiva, M. Teixeira, W. Meyer-Klaucke, M.C. Feiters and R.R. Crichton, “Comparative Fe and Zn K-edge X-ray absorption spectroscopic study of the ferroxidase centres of human H-chain ferritin and bacterioferritin from Desulfovibrio desulfuricansJ. Biol. Inorg. Chem. (2009), 14(1): 35-49.



• T.F. Oliveira, C. Vonrhein, P.M. Matias, S.S. Venceslau, I.A.C. Pereira, M. Archer, “The crystal structure of Desulfovibrio vulgaris dissimilatory sulfite reductase bound to DsrC provides novel insights into the mechanism of sulfate reduction” J. Biol. Chem. (2008), 283(49):34141-9.

• M.D. Freixo, A. Karmali, C. Frazão and J.M. Arteiro, “Production of laccase and xylanase from Coriolus versicolor grown on tomato pomace and their chromatographic behaviour on immobilized metal chelates” Process Biochemistry (2008), 43(11):1265-1274.

• T.F. Oliveira, C. Vonrhein, P.M. Matias, S.S. Venceslau, I.A.C. Pereira and M. Archer, “Purification, crystallization and preliminary crystallographic analysis of a dissimilatory DsrAB sulfite reductase in complex with  DsrC” J. Struct. Biol. (2008), 164(2):236-239.

• M. Stelter, A.M.P. Melo, M.M. Pereira, C.M. Gomes, G.O. Hreggvidsson, J.K. Kristjansson, L.M. Saraiva, M. Teixeira, M. Archer, "A novel type of monoheme cytochrome c: Biochemical and strutural characterization at 1.23 A resolution of Rhodothermus marinus cytochrome c", Biochemistry (2008), 47(46):11953-63.

• M. V. Petoukhov, J. B. Vicente, P. B. Crowley, M. A. Carrondo, M. Teixeira, D. I. Svergun, “Quaternary structure of Flavorubredoxin as revealed by synchrotron radiation small-angle X-ray scattering”, Structure (2008), 16(9):1428-36.

• S. Gorynia, P. M. Matias, T. M. Bandeiras, P. Donner and M. A. Carrondo, "Cloning, expression, purification, crystallization and preliminary X-ray analysis of the human RuvBL1–RuvBL2 complex", Acta Cryst. (2008) F64, 840–846.

•  M.L. Rodrigues, K.A. Scott, M.S.P. Sansom, I.A.C. Pereira and M. Archer, “Quinol oxidation by c-type cytochromes: structural characterization of the menaquinol binding site of NrfHA.” J. Mol. Biol. (2008), 381(2): 341-350.

• D. de Sanctis, I. Bento, J.M. Inácio, S. Custódio, I. de Sá-Nogueira, M.A.Carrondo, "Overproduction, crystallization and preliminary X-ray characterization of Abn2, an endo-1,5-alpha-arabinanase from Bacillus subtilis" Acta Cryst. (2008) F64, 636-638.

• D. Aragão, E. Mitchell, C. Frazão, M.A. Carrondo and P. Lindley “Structural and Functional Relationships in the Hybrid Cluster Protein Family: the Structure of the Anaerobically Purified Hybrid Cluster Protein from Desulfovibrio vulgaris at 1.35A Resolution”, Acta Cryst. (2008) D64, 665-674.

• P. Durão, Z. Chen, C.S. Silva, C.M. Soares, M.M. Pereira, S. Todorovic, P. Hildebrandt, I. Bento, P.F. Lindley, and L.O. Martins, "Proximal mutations at the type 1 copper site of CotA laccase: Spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants", Biochem. J. (2008), 412(2): 339-46.

• P. B. Crowley, P. M. Matias, H. Mi, S. J. Firbank, M. J. Banfield and C. Dennison, “Regulation of Protein Function: Crystal Packing Interfaces and Conformational Dimerization” Biochemistry (2008), 47(25):6583–6589.

• S.A.L. Lobo, A.A. Brindley, C.V. Romão, H.K. Leech, M.J. Warren and L.M. Saraiva, “Two distinct roles for two functional cobaltochelatases (CbiK) in Desulfovibrio vulgaris Hildenborough”, Biochemistry (2008), 47(21):5851–5857.

• D. Plácido, C. G. Fernandes, A. Isidro, M. A. Carrondo, A. O. Henriques, M. Archer “Auto-induction and purification of a Bacillus Subtilis transglutaminase (Tgl) and its preliminary crystallographic characterization”, Protein Expres. Purif., (2008), 59: 1-8.

• T. M. Bandeiras, R. C. Hillig, P. M. Matias, U. Eberspaecher, J. Fanghänel, M. Thomaz, S. Miranda, K. Crusius, V. Pütter, P. Amstutz, M. Gulotti-Georgieva, H. K. Binz, C. Holz, A. A. P. Schmitz, C. Lang, P. Donner, U. Egner, M. A. Carrondo and B. Müller-Tiemann, “Structure of Wild-Type Plk-1 Kinase Domain in Complex with a Selective DARPin” Acta Cryst. (2008) D64, 339-353.

• C. Frazão, D. Aragão, R. Coelho, S. S. Leal, C. M. Gomes, M. Teixeira and M. A. Carrondo, “Crystallographic analysis of the intact metal centres [3Fe4S]1+/0 and [4Fe4S]2+/1+ in a Zn2+-containing ferredoxin” FEBS Letters (2008), 582(5):763-767.

• A.M.D. Gonçalves, A.T. Rêgo, M. Thomaz, F.J. Enguita and M.A. Carrondo, "Expression, purification, crystallization and preliminary X-ray characterization of two crystal forms of stationary-phase survival E protein from Campylobacter jejuni" Acta Cryst. (2008) F64, 213-216.

• D. de Sanctis, A. T. Rêgo, D. Marçal, C. E. McVey, M. A. Carrondo and F. J. Enguita “Overexpression, purification and crystallization of the tetrameric form of SorC sorbitol operon regulator” Acta Cryst. (2008) F64, 22-24.

Book Chapters:

•  M.L. Rodrigues, I.A.C. Pereira and M. Archer, “The NrfH Cytochrome c Quinol Dehydrogenase”, in Handbook of Metalloproteins, Ed. A. Messerschmidt, John Wiley & Sons, Ltd. (2008), DOI: 10.1002/0470028637.met221.

• J. B. Vicente, M. A. Carrondo, M. Teixeira and C. Frazão, “Structural Studies on Flavodiiron Proteins”, in Methods in Enzymol. (2008), Globins and other Nitric Oxide-Reactive Proteins Part B, 437:3-19.

• G. Leonard, D. Nurizzo, C. Dieckmann-Mueller, E. Mitchell, J. McCarthy, D. Flot, I. Bento and P. Lindley, "Crystallographic Research Developments" in Astrophysics and Condensed Matter – Horizons in World Physics, (2008) Ed. T.G. Hardwell, Nova Science Publishers, Inc., Vol. 262, pp 11-69.



• M. Stelter, A.M.P. Melo, L.M. Saraiva, M.Teixeira and M. Archer, "Crystallization and X-Ray Analysis of Rhodothermus marinus cytochrome c at 1.23 A Resolution” Protein & Peptide Letters (2007), 14(10): 1038-1040.

• M.G. Cuypers, E.P. Mitchell, C.V. Romão and S.M. McSweeney, "The crystal structure of the Dps2 from Deinococcus radiodurans reveals an unusual pore profile with a non-specific metal binding site" J. Mol. Biol. (2007), 371(3): 787-799.

• J. Mulchande, L. Martins, R. Moreira, M. Archer, T. F. Oliveira and J. Iley, "The efficiency of C-4 substituents in activating the beta-lactam scaffold towards serine proteases and hydroxide ion" Organic & Biomolecular Chemistry (2007), 5(16): 2617-2626.

• J. Andrade, A. Karmali, M. A. Carrondo, and C. Frazão “Structure of amidase from Pseudomonas aeruginosa showing a trapped acyl transfer reaction intermediate state”, J. Biol. Chem. (2007), 282: 19598-605.

• J. Tatur, W.R. Hagen and P. M. Matias, “Crystal structure of the ferritin from the hyperthermophilic archaeal anaerobe Pyrococcus furiosus”, J. Biol. Inorg. Chem. (2007), 12(5):615-630.

• D. Aragão, A.M. Fialho, A.R. Marques, E.P. Mitchell, I. Sá-Correia and C. Frazão, "The complex of Sphingomonas elodea ATCC 31461 glucose-1-phosphate uridylyltransferase with glucose-1-phosphate reveals a novel quaternary structure, unique among nucleoside diphosphate-sugar pyrophosphorylase members" J. Bacteriol. (2007), 189(12): 4520-4528.

• S. Cooray, M.W. Bahar, N.G. Abrescia, C.E. McVey, N.W. Bartlett, R.A. Chen, D.I. Stuart, J.M. Grimes, G.L. Smith, "Functional and structural studies of the vaccinia virus virulence factor N1 reveal a Bcl-2-like anti-apoptotic protein", J. Gen. Virol. (2007), 88(6):1656-66.

• M. A. Carrondo, I. Bento, P. M. Matias and P. F. Lindley "Crystallographic evidence for dioxygen interactions with iron proteins", J. Biol. Inorg. Chem. (2007), 12: 429-442.

• D. Marçal, A. T. Rêgo, M. J. Fogg, K. S. Wilson, M. A. Carrondo and F. J. Enguita "Crystallization and preliminary X-ray characterization of 1,3-propanediol dehydrogenase from the human pathogen Klebsiella pneumoniae" Acta Cryst. (2007) F63, 249-251.

• J. Andrade, A. Karmali, M. A. Carrondo and C. Frazão “Crystallization, diffraction data collection and preliminary crystallographic analysis of hexagonal crystals of Pseudomonas aeruginosa amidase”, Acta Cryst. (2007) F63, 214-216.

• I. Bento, C. Peixoto, V. N. Zaitsev and P. F. Lindley. "Ceruloplasmin revisited: structural and functional roles of various metal cation-binding sites" Acta Cryst. (2007) D63, 240-248.

• T.F. Oliveira, J. Mulchande, R. Moreira, J. Iley and M. Archer “Crystallization and Preliminary Diffraction Studies of Porcine Pancreatic Elastase in Complex with a Novel Inhibitor”, Protein & Peptide Letters (2007), 14(1): 93-95.



• P. M. Matias, S. Gorynia, P. Donner, M. A. Carrondo, “Crystal structure of the human AAA+ protein RuvBL1” J. Biol. Chem. (2006), 281: 38918-29.

• C.C. Almeida, C.V. Romão, P.F. Lindley, M. Teixeira and L.M. Saraiva “The role of the hybrid cluster protein in oxidative stress defense” J. Biol. Chem. (2006), 281: 32445-50.

• M. L. Rodrigues, T. Oliveira, I. A. C. Pereira and M. Archer “X-ray structure of a membrane-bound cytochrome c quinol dehydrogenase with novel heme coordination” EMBO J. (2006), 25: 5951-60.

• A.V. Lyashenko, I. Bento, V.N. Zaitsev, N.E. Zhukhlistova, Y.N. Zhukova, A.G. Gabdoulkhakov, E.Y. Morgunova, W. Voelter, G.S. Kachalova, E.V. Stepanova, O.G. Koroleva, V.S. Lamzin, V.I. Tishkov, C. Betzel, P.F Lindley, A.B. Mikhailov. “X-ray structural studies of the fungal laccase from Cerrena maxima”, J. Biol. Inorg. Chem. (2006), 11: 963-973.

• F.C. Teixeira, H. Ramos, I.F. Antunes, M.J. Curto, M.T. Duarte, I. Bento, “Synthesis and structural characterization of 1- and 2-substituted indazoles: ester and carboxylic acid derivatives” Molecules. (2006), 11(11):867-89.

• M.J. Fogg, P. Alzari, M. Bahar, I. Bertini, J. M. Betton, W. P. Burmeister, C. Cambillau, B. Canard, M. Carrondo, M. Coll, S. Daenke, O. Dym, M. P. Egloff, F. J. Enguita, A. Geerlof, A. Haouz, T. A. Jones, Q. Ma, S. N. Manicka, M. Migliardi, P. Nordlund, R. J. Owens, Y. Peleg, G. Schneider, R. Schnell, D. I. Stuart, N. Tarbouriech, T. Unge, A. J. Wilkinson, M. Wilmanns, K. S. Wilson, O. Zimhony and J. M. Grimes, “Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens”, Acta Cryst. (2006) D62, 1196-1207.

• A. Geerlof, J. Brown, B. Coutard, M. P. Egloff, F. J. Enguita, M. J. Fogg, R.J.C. Gilbert, M.R. Groves, A. Haouz, J. E. Nettleship, P. Nordlund, R. J. Owens, M. Ruff, S. Sainsbury, D.I. Svergun and M. Wilmanns, "The impact of protein characterization in structural proteomics" Acta Cryst. (2006) D62, 1125-1136.

• A.V. Lyashenko, N.E. Zhukhlistova, A.G. Gabdoulkhakov, Y.N. Zhukova, W. Voelter, V.N. Zaitsev, I. Bento, E.V. Stepanova,  G.S. Kachalova, O.V. Koroleva, E.A. Cherkashyn, V.I. Tishkov, V.S. Lamzin, K. Schirwitz, E.Y. Morgunova, C. Betzel, P.F. Lindley,  A.M. Mikhailov. “Purification, crystallization and preliminary X-ray study of the fungal laccase from Cerrena maxima.”, Acta Cryst. (2006) F62, 954-7.

• D. Aragão, A.R. Marques, C. Frazão, F.J. Enguita, M.A. Carrondo, A.M. Fialho, I. Sa-Correia, E.P. Mitchell "Cloning, expression, purification, crystallization and preliminary structure determination of glucose-1-phosphate uridylyltransferase (UgpG) from Sphingomonas elodea ATCC 31461 bound to glucose-1-phosphate", Acta Cryst. (2006) F62, 930-934.

• C. Frazão, C. E. McVey, M. Amblar, A. Barbas, C. Vonrhein, C. M. Arraiano and M.A. Carrondo “Unravelling the dynamics of RNA degradation by RNAse II and its RNA- bound complex”, Nature (2006), 443: 110-114.

• D. de Sanctis, P. Ascenzi, A. Bocedi, S. Dewilde, T. Burmester, T. Hankeln, L. Moens, M. Bolognesi "Cyanide binding and heme cavity conformational transitions in Drosophila melanogaster hexacoordinate hemoglobin." Biochemistry (2006), 45: 10054-61.

• I. Bento, M. A. Carrondo, P. F. Lindley, “Reduction of dioxygen by enzymes containing copper”, J. Biol. Inorg. Chem. (2006), 11: 539-547.

• C.E. McVey, M. Amblar, A. Barbas, F. Cairrão, R. Coelho, C. Romão, C.M. Arraiano, M.A. Carrondo and C. Frazão “Expression, purification, crystallization and preliminary diffraction data characterization of Escherichia coli ribonuclease II (RNase II).”, Acta Cryst. (2006) F62, 684-687.

• P.N. Palma, M. L. Rodrigues, M. Archer, M. J. Bonifácio, A. I. Loureiro, D. Learmonth, M. A. Carrondo and P. Soares-da-Silva, “Comparative study of ortho- and meta- nitrated inhibitors of catechol-O-methyltransferase. Interactions with the active site and regioselectivity of O-methylation” Molecular Pharmacology (2006), 70: 143-153.

• A.L. Simplício, P. M. Matias, J. F. Gilmer, J.M. Clancy “Chiral separation and identification of beta-aminoketones of pharmacological interest by high performance liquid chromatography and capillary electrophoresis”, J. Chromatography A. (2006), 1120: 89-93.

• M. L. Rodrigues, T. Oliveira, P. M. Matias, I. C. Martins, F. M. A. Valente, I. A. C. Pereira and M. Archer “Crystallization and preliminary structure determination of the membrane-bound complex cytochrome c nitrite reductase from Desulfovibrio vulgaris Hildenborough”. Acta Cryst. (2006) F62, 565-568.

• P. Durão, I. Bento, A.T. Fernandes, E.P. Melo, P.F. Lindley, L.O. Martins. “Perturbations of the T1 copper site in the CotA laccase from Bacillus subtilis: structural, biochemical, enzymatic and stability studies” J. Biol. Inorg. Chem. (2006), 11: 514-26.

• J. Lachwa, I. Bento, M.T. Duarte, J.N. Lopes, L.P. Rebelo. “Condensed phase behaviour of ionic liquid-benzene mixtures: congruent melting of a [emim][NTf2].C6H6 inclusion crystal” Chem. Commun. (2006), 23: 2445-7.

• J. A. Brito, T. M. Bandeiras, M. Teixeira, C. Vonrhein and M. Archer, “Crystallisation and preliminary structure determination of a NADH:quinone oxidoreductase from the extremophile Acidianus ambivalens,” Biochimica et Biophysica Acta – Proteins and Proteomics (2006), 1764(4): 842-845.

• F.J. Enguita, E. Pohl, D.L. Turner, H. Santos, M.A. Carrondo, "Structural evidence for a proton transfer pathway coupled with haem reduction of cytochrome c'' from Methylophilus methylotrophus" J. Biol. Inorg. Chem. (2006), 11: 189-196.

• T. Ulrich, C. M. Gomes, A. Kletzin and C. Frazão "X-ray structure of a self-compartmentalizing sulfur cycle metalloenzyme", Science (2006), 311: 996-1000.

• M.L. Rodrigues, M. Archer, P. Martel, S. Miranda, M. Thomaz, F.J. Enguita, R.P. Baptista, E. Pinho e Melo, N. Sousa, A. Cravador, M.A. Carrondo, "Crystal structures of the free and sterel-bound forms of b-cinnamomin", Biochimica et Biophysica Acta – Proteins and Poteomics (2006), 1764(1): 110-121.

• S. Gorynia, P.M. Matias, S. Gonçalves, R. Coelho, G.L. Gato, M. Thomaz, M. Huber, B. Haendler, P. Donner and M.A. Carrondo, "Expression, purification, crystallization and preliminary X-ray analysis of the human RuvB-like protein RuvBL1" Acta Cryst. (2006) F62, 61-66.



• I. Bento, L. O. Martins, G. G. Lopes, M. A. Carrondo and P. F. Lindley “Dioxygen reduction by multi-copper oxidases; a structural perspective”, Roy. Chem. Soc. Dalton Trans. (2005), 21:3507-3513.

• P. M. Matias, I. A. C. Pereira, C. M. Soares and M. A. Carrondo “Sulphate respiration from hydrogen in Desulfovibrio bacteria: a structural biology overview”, Progress in Biophysics and Molecular Biology (2005), 89: 292-329.

• A. Athanasiadis, D. Placido , S. Maas, B.A. Brown , K. Lowenhaupt , A. Rich “The crystal structure of the Z-beta domain of the RNA-editing enzyme ADAR1 reveals distinct conserved surfaces among Z-domains”, J. Mol. Biol. (2005), 351: 496-507.

• P.M. Matias, J. Tatur, M.A. Carrondo and W.R. Hagen, "Crystallization and preliminary X-ray characterization of a ferritin from the hyperthermophilic archaen and anaerobe Pyrococcus furious" Acta Cryst. (2005) F61, 503-506.

• P. B. Crowley and A. Golovin, "Cation-pi interactions in protein-protein interfaces", Proteins: Structure, Function and Bioinformatics (2005), 59(2):231-9.

• K. Sato, P. B. Crowley and C. Dennison, "Transient homodimer interactions studied using the electron self-exchange reaction", J. Biol. Chem. (2005), 59: 265-7.

• T. Urich, R. Coelho, A.f Kletzin, C. Frazão, "The sulfur oxygenase reductase from Acidianus ambivalens is an icosatetramer as shown by crystallization and Patterson analysis", Biochimica et Biophysica Acta – Proteins and Proteomics (2005), 1747(2):267-70.

• I. M. Díaz-Moreno, A. Díaz-Quintana, M. A. de la Rosa, P. B. Crowley and M. Ubbink, "Different modes of interaction in cyanobacterial complexes of plastocyanin and cytochrome f", Biochemistry (2005), 44(9):3176-83.

• C. Frazão, D.C. Simes, R. Coelho, D. Alves, M.K. Williamson, P.A. Price, M.L. Cancela and M.A. Carrondo “Structural evidence of a fourth Gla residue in fish osteocalcin: Biological implications” Biochemistry (2005), 44: 1234-1242.

• M. L. Rodrigues, M. J. Bonifácio, P. Soares-da-Silva, M. A. Carrondo, M. Archer “Crystallization and preliminary X-ray diffraction studies of a catechol-O- methyltransferase/inhibitor complex”, Acta Cryst. (2005) F61, 118-120.



• I. J. Correia, C. M. Paquete, A. Coelho, C. C. Almeida, T. Catarino, R. O. Louro, C. Frazão, L. M. Saraiva, M. A. Carrondo, D. L. Turner and A. V. Xavier, “Proton assisted two-electron transfer in natural variants of tetraheme cytochromes from Desulfomicrobium sp.” J. Biol. Chem. (2004), 279: 52227-37.

• F. J. Enguita, D. Marçal, L. O. Martins, R. Grenha, A. O. Henriques, P. F. Lindley and M. A. Carrondo "Substrate and dioxygen binding to the endospore coat laccase from Bacillus subtilis", J. Biol. Chem. (2004), 279: 23472-23476.

• P. Crowley and M.A. Carrondo, “The Architecture of the Binding Site in Redox Protein Complexes: Implications for Fast Dissociation”, Proteins: Structure, Function and Bioinformatics (2004), 55: 603-612.

• I. Bento, P. M. Matias, A. M. Baptista, P. N. da Costa, W. M. A. M. van Dongen, L. M. Saraiva, T. R. Schneider, C. M. Soares and M. A. Carrondo “Molecular basis for redox-Bohr and cooperative effects in cytochrome c3 from Desulfovibrio desulfuricans ATCC 27774: crystallographic and modelling studies of oxidised and reduced high-resolution structures at pH 7.6” Proteins: Structure, Function and Bioinformatics (2004), 54: 135-152.



• S. Macedo, D. Aragão, E. P. Mitchell and P. Lindley “Structure of the hybrid cluster protein (HCP) from Desulfovibrio desulfuricans ATCC 27774 containing molecules in the oxidized and reduced states” Acta Cryst. (2003) D59, 2065-2071.

• I. Bento, V. H. Teixeira, A. M. Baptista, Cláudio M. Soares, P. M. Matias and M. A. Carrondo “Redox-Bohr and other cooperativity effects in the nine heme cytochrome c from Desulfovibrio desulfuricans ATCC 27774: crystallographic and modeling studies”. J. Biol. Chem. (2003), 278: 36455-36469.

• F. J. Enguita, L. O. Martins, A. O. Henriques and M. A. Carrondo “Crystal structure of a bacterial endospore coat component: a laccase with enhanced thermostability properties” J. Biol. Chem. (2003), 278: 19416-19425.

• M. A. Carrondo “Ferritins, Iron Uptake and Storage from the Bacterioferritin Viewpoint” EMBO J. (2003), 22: 1959-1968.

• D. Aragão, S. Macedo, E. P. Mitchell, C. V. Romão, M. Y. Liu, C. Frazão, L. M. Saraiva, A. V. Xavier, J. LeGall, W. M.A.M. van Dongen, W. R. Hagen, M. Teixeira, M. A. Carrondo and P. Lindley “Reduced Hybrid Cluster Proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough). X-ray Structures at High Resolution using Synchrotron Radiation”, J. Biol. Inorg. Chem. (2003), 8: 540-548.

• S. Macedo, C. V. Romão, E. Mitchell , P. M. Matias, M. Y. Liu , A. V. Xavier , J. LeGall , M. Teixeira, P. Lindley and M. A. Carrondo “The nature of the di-iron site in the bacterioferritin from Desulfovibrio desulfuricansNature Struct. Biol (2003), 10: 285-290.

• D. Aragão, C. Frazão, L. Sieker, G. M. Sheldrick, J. LeGall, and M. A. Carrondo “Crystal structure of the di-tetraheme cytochrome c3 from Desulfovibrio gigas at 1.2 Å resolution” Acta Cryst. (2003) D59, 644-653.

• F. J. Enguita, L. Rodrigues, M. Archer, L. Sieker, A. Rodrigues, D. L. Turner, H. Santos and M. A. Carrondo “Crystallization and Preliminary X-Ray Characterization of Cytochrome c” from the obligate methylotroph Methylophilus methylotrophusActa Cryst. (2003) D59, 580-583.

• I. A. Abreu, A. I. Lourenço, A. V. Xavier, J. LeGall, A. V. Coelho, P. M. Matias, D. M. Pinto, M. A. Carrondo, M. Teixeira and L. M. Saraiva “A novel iron centre in the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774” J. Biol. Inorg. Chem. (2003), 8: 360-370.



• P. M. Matias, A. V. Coelho, F. M. A. Valente, D. Plácido, J. LeGall, A. V. Xavier, I. A. C. Pereira and M. A. Carrondo “Sulfate respiration in Desulfovibrio vulgaris Hildenborough: Structure of the 16-heme Cytochrome c HmcA at 2.5 Å resolution and a view of its role in transmembrane electron transfer” J. Biol. Chem. (2002), 277(49): 47907-47916.

• M. J. Bonifácio, M. Archer, M. L. Rodrigues, P. M. Matias, D. A. Learmonth, M. A. Carrondo and P. Soares-da-Silva “Kinetics and Crystal Structure of Catechol-O-Methyltransferase Complex with Co-Substrate and a Novel Inhibitor with Potential Therapeutic Application” Molecular Pharmacology (2002), 62: 795-805.

• F.J. Enguita, P.M. Matias, L.O. Martins, D. Plácido, A. O. Henriques and M.A. Carrondo “Spore coat Laccase CotA from Bacillus subtilis: Crystallization and Preliminary X-Ray Characterization by the MAD method" Acta Cryst. (2002) D58, 1490-1493.

• M. L. Rodrigues, M. Archer, P. Martel, A. Jacquet, A. Cravador, and Maria A. Carrondo, “Crystal structure of beta-cinnamomin, a toxic protein for some plant species”, Acta Cryst. (2002) D58, 1314-1321.

• S. Macedo, E. P. Mitchell, C. V. Romão, S. J. Cooper, R. Coelho, M. Y. Liu, A. V. Xavier, J. LeGall, S. Bailey, C. D. Garner, W. R. Hagen, M. Teixeira, M.A. Carrondo and P. Lindley, “Hybrid cluster proteins (HCPs) from Desulfovibrio desulfuricans ATCC 2774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at 1.25 Å resolution using synchrotron radiation” J. Biol. Inorg. Chem. (2002), 7:514-525.

• C. M. Gomes, C. Frazão, A. V. Xavier, J. Legall and M. Teixeira "Functional control of the binuclear metal site in the metallo-beta-lactamase-like fold by subtle amino acid replacements" Protein Science (2002), 11:707-712.

• P. M. Matias, M. A. Carrondo, R. Coelho, M. Thomaz, X.-Y. Zhao, A. Wegg, K. Crusius, U. Egner, and P. Donner “Structural basis for the glucocorticoid response in a mutant human androgen receptor (ARccr) derived from an androgen-independent prostate cancer”, J. Med. Chem. (2002), 45: 1439-1446.



• R. O. Louro, I. Bento, P. M. Matias, T. Catarino, A. M. Baptista, C. M. Soares, M. A. Carrondo, D. L. Turner, A. V. Xavier, “Conformational component in the coupled transfer of multiple electrons and protons in a monomeric tetraheme cytchrome” J. Biol. Chem. (2001), 276: 44044-44051.

• J. Mendes, A.M. Baptista, M.A. Carrondo, C.M. Soares, "Implicit solvation in the self-consistent mean field theory method: sidechain modelling and prediction of folding free energies of protein mutants", J. Comp. Aid. Mol. Des. (2001), 15: 721-740.

• J. Mendes, H. Nagarajaram, C.M. Soares, T. L. Blundell and M.A. Carrondo “Incorporating knowledge-based biases into an energy-based side-chain modeling method: application to comparative modeling of protein structure”, Biopolymers (2001), 59:72-86.

• M. L. Rodrigues, M. Archer, M. J. Bonifácio, P. Soares-da-Silva and M. A. Carrondo “Crystallisation and preliminary crystallographic characterisation of catechol-O-methyltransferase in complex with its co-substrate and an inhibitor” Acta Cryst. (2001) D57, 906-908.

• D. A. Learmonth, J. Benes, A. Parada, D. Hainzl, A. Beliaev, M. J. Bonifácio, P. M. Matias, M. A. Carrondo, J. Garret and P. Soares-da-Silva “Synthesis, anticonvulsant properties and pharmacokinetic profile of novel 10,11-dihydro-10-oxo-5H-dibenz/b,f/azepine-5-carboxamide derivatives” Eur. J. Med. Chem. (2001), 36: 227-236.

• C. Frazão, F. J. Enguita, R. Coelho, G. M. Sheldrick, J. A. Navarro, M.Hervás, M. A. De la Rosa, M. A. Carrondo “Crystal structure of low-potential cytochrome c549 from Synechocystis sp. PCC 6803, at 1.21 Å resolution” J. Biol. Inorg. Chem. (2001), 6:324-332.

• A.V. Coelho, S. Macedo, P. M. Matias, A. W. Thompson, J. LeGall and M.A. Carrondo “Structure determination of Bacterioferritin from Desulfovibrio desulfuricans by the MAD method at the Fe K-edge” Acta Cryst. (2001) D57, 326-329.

• P.M. Matias, C. M. Soares, L. M. Saraiva, R. Coelho, J. Morais, J. Le Gall and M.A. Carrondo, “[NiFe] Hydrogenase from Desulfovibrio desulfuricans ATCC 27774: Gene sequencing, three-dimensional structure determination and refinement at 1.8 Å and modelling studies of its interaction with the tetra-haem cytochrome c3” J. Biol. Inorg. Chem. (2001), 6: 63-81.

Book Chapters:

• M. A. Carrondo, C. M. Soares, P.M. Matias “Nine-heme Cytochrome c”, in Handbook of Metalloproteins, Ed. K. Wieghardt, R. Huber, T. L. Poulos, A. Messerschmidt, John Wiley & Sons, Ltd. (2001), pp 147-158.


• P. Donner, U. Egner, M. A. Carrondo and P.M. Matias ”Crystal” World Intellectual Property Organization, International Publication Number WO 01/66599 A1, publication date 13/09/2001.

Pedagogical publications:

• C. Frazão, C. M. Gomes, G. Silva, S. Oliveira, A.V. Xavier, J. LeGall, M. Teixeira and M. A. Carrondo “ Utilização de oxigénio em organismos anaeróbicos – Estrutura da rubredoxina:oxigénio oxidoreductase, o protótipo de uma nova família de metaloenzimas” Boletim de Biotecnologia (2001), 70: 2-6.



• C. Frazão, G. Silva, C. M. Gomes, P. M. Matias, R. Coelho, L. Sieker, S. Macedo, M. Y. Liu, S. Oliveira, M. Teixeira, A. V. Xavier, C. Rodrigues-Pousada, M. A. Carrondo, and J. Le Gall, “Structure of a dioxygen reduction enzyme from Desulfovibrio gigas”, Nature Struct. Biol. (2000), 7: 1041-1045.

• P.M. Matias, P. Donner, R. Coelho, M. Thomaz, C. Peixoto, S. Macedo, N. Otto, S. Joschko, P. Scholz, A. Wegg, S. Bäsler, M. Schäfer, U. Egner and M.A. Carrondo, “Structural evidence for ligand specificity in the binding domain of the human Androgen receptor: implications for phatogenic gene mutations” J. Biol. Chem. (2000), 275: 26164-26171.

• M. Archer, M.L. Rodrigues, M. Aurélio, R. Biemans, A. Cravador and M.A. Carrondo, “Crystallisation and preliminary X-ray diffraction analysis of beta-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomin”, Acta Cryst. (2000) D56, 363-365.


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Macromolecular Crystallography Unit 2016

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